The Cell Biology of Stress | Pepperdine University | Seaver College

The Cell Biology of Stress

First-Year Student as Scholars Seminar

Dr. Jay Brewster, Professor of Biology, Pepperdine University

Cell biology of stress: Pepperdine student in lab

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With increasing industrialization and human population growth, air pollution has become an environmental threat to human health. Students in this seminar will expose human cells in tissue culture to nanoparticles found in polluted air as well as improvise simple methods to assess cellular stress, survival, and biochemical activity. Methods of analyses will include light microscopy, immunohistochemistry, immunoblot analysis of signaling proteins, and assays for metabolic function.

Cell Signaling

Examples of Student Research

Presentations by Undergrads at Scientific Conferences

Students denoted by *

Jones*, C. , A. Calixto, B. Manes, E. Blair, S. Alvarado, and J.L. Brewster. 2015. Nanoparticle-induced stress eladds to elevated reactive oxygen species, punctate mitochondria, and apoptosis in human lung cells. Southern California Conferences for Undergraduate Research. OS-3-4:00.

Jones*, C.Y., A. Calixto, B. Manes, S.K. Alvarado, J.L. Brewster. 2015. Nanoparticle-induced stress leads to elevated reactive oxygen species, punctate mitochondria, and apoptosis in human lung cells. Southern California Conferences for Undergraduate Research. OS3-B470-4:00.

Manes* B., S. Alvarado*, and J.L. Brewster 2013. Nanoparticle exposure activates an inositol trisphosphate receptor-dependent elevation of reactive oxygen species and apoptosis in human alveolar lung cells, American Society for Cell Biology Annual Meeting (New Orleans, LA), Session: Proteostasis, Cell Stress, and Aging, Abstract 1470.

Mercado* V., and J.L. Brewster. 2013. The surviving and clAP1 anti-apoptotic proteins are differentially down regulated in response to endoplasm reticulum stress. Southern California Conferences for Undergraduate Research. Abstract OS2-20.

Mercado* V., and J.L. Brewster 2013. The Survivin and cIAP1 anti-apoptotic proteins are destabilized during endoplasmic reticulum stress, American Society for Cell Biology Annual Meeting (New Orleans, LA), Session: Proteostasis, Cell Stress, and Aging, Abstract 1472.

Vargas* A., and J.L. Brewster. 2013. Assessment of BHK-21 cell viability at low and high concentrations of Tunicamycin after transfection of Bcl-2 ER localized anti-apoptotic protein. Southern California Conferences for Undergraduate Research. Abstract PSI-9.

Manes* B., Alvarado* S., and J.L. Brewster. 2012. Nanoparticle accumulation in human alveolar cells induce reactive oxygen species and ER stress signaling, American Society for Cell Biology Annual Meeting (San Franciso CA), Session: Proteostasis, Cell Stress, and Aging, Poster

Scholl* A., Osborne* A., Lumley* E., Scott* J., and J.L. Brewster. 2012. Moderate ER stress induces p44/p42 kinase activation and an ITPR-dependent apoptosis, American Society for Cell Biology Annual Meeting (San Franciso CA), Session: Proteostasis, Cell Stress, and Aging, Poster.

Select Recent Publications by Undergraduate Students

Students denoted by *

 

Murphy* L.A., E.A. Ramirez*, V.T. Trinh*, A.M. Herman*, V.C. Anderson*, and J.L. Brewster 2011. "Endoplasmic reticulum stress or mutation of an EF-hand Ca2+-binding domain directs the FKBP65 rotamase to an ERAD-based proteolysis", Cell Stress and Chaperones, 16:607-619.

Loucks F.A., E.K. Shcroeder, A. Zommer, S. Hilger*, N.A. Kelsey, R.J. Bouchard, C. Blackstone, J.L. Brewster, D.A. Linseman. 2009. Caspases indirectly regulate cleavage of the mitochondrial fusion GTPase OPA1 in neurons undergoing apoptosis, Brain Research, 1250:63-74.

Brewster, J.L., D.A. Linseman, R.J. Bouchard, F.A. Loucks, T.A. Precht, E.A. Esch* and K.A. Heidenreich. 2006. Endoplasmic reticulum stress and tropic factor withdrawal activate distinct signaling cascades that induce glycogen synthase kinase-3b and a caspase-9-dependent apoptosis in cerebellar granule neurons. Molecular and Cellular Neuroscience 32:242-253.

Niederer*, K., D. Morrow*, J. Gettings*, M. Irick*, A. Krawiecki* and J.L. Brewster. 2005. Cypermethrin blocks a mitochondria-dependent apoptotic signal initiated by deficient N-linked glycosylation within the endoplasmic reticulum, Cellular Signalling 17 (2):177-186.

Pepperdine student presents research

Pepperdine students in front of poster

Pepperdine student sharing research and poster